an R package for the simple simulation of single-cell RNA sequencing data. This vignette gives an overview and introduction to Splatter’s functionality. Long Description (required) As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets. Unfortunately, current simulations are often poorly documented, their similarity to real data is not demonstrated, or reproducible code is not available. Here, we present the Splatter Bioconductor package for simple, reproducible, and well-documented simulation of scRNA-seq data. Splatter provides an interface to multiple simulation methods including Splat, our own simulation, based on a gamma-Poisson distribution. Splat can simulate single populations of cells, populations with multiple cell types, or differentiation paths. RNA-seq; Simulation; Single-cell; Software https://bioconductor.org/packages/devel/bioc/vignettes/splatter/inst/doc/splatter.html Step 1: Use the attribute tree to add new attributes or remove pre-selected attributes to describe the simulator. Every sub-attribute is selected Not all sub-attributes are selected Fill Clear Expand Collapse Reset Summary of Proposed Changes Step 2: Review list of proposed attribute addition(s) and subtraction(s). Can't Find the Attribute You Are Looking For?
If you would like to propose an attribute that you cannot find in the tree above, or if you would like to add a clarification to one or more attributes for this simulator (e.g. a specific file format for attribute /Output/File Format/Other), please list them in the
Additional Comment box of the Submit tab. Summary of Proposed Changes Current Citations/Applications
Splatter: simulation of single-cell RNA sequencing data. Genome Biol,
https://www.ncbi.nlm.nih.gov/pubmed/?term=28899397, Primary Citation